Please what is the best solvent to dissolve heme?

I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]

04 January 2021 5,528 2 View

PVDF membrane degradation?

Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...

18 November 2020 6,412 7 View

What to do when you found a published paper that doesn't belong to you but has your name as the author?

I found a published paper online (attached here) that bears mine and colleague's names as authors but the paper doesn't belong to us. The work was claimed to be carried out in Kenya. We are in...

22 March 2020 4,860 7 View

Do you know a (validated) instrument for the assessment of state embarassment?

I've found quite a few scales for vicarious and personal embarassibility as a personality trait so far (i. e. Kelly & Jones, 1997; Maltby & Day, 2000; Uysal et al., 2014...). As I'd like...

24 February 2020 8,142 4 View

Are Peak Areas of different silylations comparable?

If I have an analyte, that has different silylations, like 2-TMS and 3-TMS, how can I compare the peak areas? Can I simply sum them up? What if theres a different ratio of 2-TMS/3-TMS in my...

03 December 2019 273 4 View

What is a good topic for Blockchain applications in IoT?

Hello everyone, at the moment I'm looking for a topic for my bachelor thesis. I was thinking about Blockchain and Smart Contracts for cyber-physical Systems, in order to use it for Smart Factory...

23 October 2019 3,694 5 View

How to carry out western blotting for a 233 kDa protein effectively?

Hi, I want to carry out western blotting for a 233 kDa protein. I was told to use a tris-acetate gel due to the size of the protein. However, I was wandering would I be able to detect the...

25 March 2019 6,308 4 View

How to set up Coupled Displacement Boundary Conditions in COMSOL?

Dear Community, I recently switched from ANSYS Mechanical to COMSOL Multiphysics. So far I was able to transfer my models to the new platform but I yet miss one feature of ANSYS in COMSOL. I hope...

29 January 2019 4,937 3 View

SpeedVac of DNA and salt concentrations: downstream effects?

I often use a speed vacuum concentrator to concentrate my DNA prior to restriction digests, DNA ligations, NGS library preparation or target captures. Usually, my DNA is eluted in 10 mM Tris-Cl...

13 November 2018 6,049 3 View

How do I calculate NDVI images with different thresholds using Sentinel-2 image?

I want to set different thresholds for calculating the NDVI images using Sentinel-2 for the purposes of fishing out river sediments in the Dry and Wet seasons. I am using the Semi-automatic...

05 November 2018 694 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

Which regression is best fit when moderator is categorical and dependent variable is latent in nature?

I am using categorical variables as a moderator variable, and my dependent, mediator and IV variables are latent in nature. Which regression test I can apply. As logistic regression is for binary...

11 February 2021 1,584 3 View

Cause of slow bromophenol blue migration and smearing while running SDS PAGE?

While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...

08 February 2021 5,317 3 View

QPCR troubleshooting - what could cause an increasing ROX signal?

Hi everyone, I'm looking for some help troubleshooting a recent qPCR issue I've encountered. I am using fluorescent Taqman probes and a qPCR master mix that includes ROX as a passive reference....

08 February 2021 419 3 View

Professional editing and proofreading services?

08 February 2021 7,901 6 View

How to avoid polypyrrole deposit on counter electrode?

Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...

05 February 2021 2,103 3 View

Can anyone help me in this MATLAB Coding?

Hello, I have a dataset excel CSV sheet with a three columns,(X,Y, and Z) The first and second columns represents the coordinates of point, while the third one represents the elevations value of...

04 February 2021 3,451 3 View