ACN polymerization is a known issue for check-valve problems, but perhaps also an interference issue in LC-MS. Any good references or experiences out there ???
Add 10% water to your ACN. Can't comment on LC-MS interference as I have never noticed it.
Polymerization of acetonitrile via a hydrogen transfer reaction from CH3 to CN only happened under extreme conditions. For example, under high pressure 1.7 GPa-20.6 GPa (246564.2 psi ~ 2987777.4 psi) or above.
https://onlinelibrary.wiley.com/doi/epdf/10.1002/anie.201606198
I have never noticed it as an interference issue in LC-MS.
Thanks for input. Its pretty established that ACN may cause a deposit of polymer on the seats of check valves. See an interesting discussion byJohn Dolan here:
http://www.chromatographyonline.com/check-valves-and-acetonitrile
I was just wondering if such polymerization could also become an interference problem in LC-MS, and if the polymer is a batch issue or could be formed in the bottle by storage.
I have seen ACN put a polymerized ring on the inner surface of a glass bottle that had sit far too long at room temperature and pressure. Polymerization in a quaternary valve was pretty much expected and frequent since we did not add water to our ACN.
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