Zulfaquar Ahmad Arfi

4 Questions 12 Answers 0 Followers

Questions related from Zulfaquar Ahmad Arfi

Can anyone provide a good reference to support the statement of minimum value which can be taken into consideration for Antiserum titer determination?

Antierum titer determination is usually done by its serial dilution and then taking the lowest positive value in comparison to blank. People take minimum twice or thrice of blank as positive...

09 January 2015 4,136 2 View

What is good titer in the case of antibodies?

In antibody titer determination, we generally compare the signal to noise ration. I guess 2:1 or 3:1 is taken as a positive titer. Is this also referred to as good titer? Are there any references...

29 November 2014 9,378 4 View

How is it possible that polyclonal antibodies produced in a rabbit shows auto cross reactivity?

Antibodies were raised against the whole cell extract.

25 April 2014 4,842 0 View

Peptide isolation

My sample contains a peptide which is acidic in nature of very very low concentration and with not known sequence. How can I isolate it and identify it. I already used SEC and IOn exchange to...

25 February 2012 3,566 7 View