Zulfa Nasir

4 Questions 1 Answers 0 Followers

Questions related from Zulfa Nasir

How I can enhance my protein expression in E.coli BL21(DE3) ? How I can increase yield? How I can reduce non-specific bands on SDS-PAGE ?

I am working with leishmania threonine synthase (LdTS) enzyme of aspartic acid pathway which is ~75kDa protein. I purified the protein and confirmed by western blot too. The condition used for...

26 March 2024 7,955 3 View

How I can enhance my protein expression in E.coli BL21(DE3) ? How I can increase yield?

Hey there, currently I am working on threonine synthase enzyme of Leishmania. I have successfully cloned it into E.coli DH5alpha strain. I have also transformed it into E.coli BL21(DE3) strain. I...

03 February 2024 1,854 4 View

How I can enhance my protein expression in E.coli BL21(DE3) ? How I can increase yield?

Hey there, currently I am working on threonine synthase enzyme of Leishmania. I have successfully cloned it into E.coli DH5alpha strain. I have also transformed it into E.coli BL21(DE3) strain. I...

03 February 2024 6,403 3 View

Has Q5 DNA polymerase ever been used in gene cloning of Leishmania?

Not been able to find any article on this. Help me to find out.

10 November 2023 1,508 1 View