Could anybody recommend me an efficient troubleshooting method in the case of Phusion or Pfu polymerase?

I would like to perform an overlap PCR. I was able to synthetize one of fragments but not the other one. I tried it with Pfu and Phusion polymerase as waell. Although  worked with Taq (annelation:...

13 August 2016 8,896 2 View