How to extract large-sized plasmids(＞50kb) from E.coli(epi300)?Traditional method or any useful kit?

Now we are working on extracting large-sized plasmids(55kbp,70kbp,100kbp) from E.coli EPI300 by traditional DNA ethanol precipitation method without the spin column. But we can't get good results...

08 January 2024 1,033 5 View