Ziaur Rehman

4 Questions 10 Answers 0 Followers

Questions related from Ziaur Rehman

Cell Cycle Assay shows no difference?

I am working on primary mice sertoli cells. I supplemented sertoli cells with different concentraions of a hormone for 48hrs. That drug cause apoptosis in conc. dependent manner check by both...

18 December 2016 5,513 5 View

Knockdown experiment in primary cells...?

Hi every body, I have the following queries please guide me in simple and step wise, 1) Which type of gene is required for knowdown experiment in cells, I mean how to select a gene? 2) How to...

24 April 2016 3,576 3 View

Sertoli cell culture - can anyone help?

I am working on male mice. I have the difficulty to culture sertoli cells from adult mice. As sertoli cells no. become fixed in adult. I used percoll gradient method and use lectin but was not...

30 April 2014 9,233 3 View

Can someone advise on a problem in sertoli cell culture, which had been contaminated with fungus on the 2nd day?

I had checked all chemicals but did not find the solution.

15 February 2014 6,935 1 View