Zhuang Xinyu

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Questions related from Zhuang Xinyu

3‘ modification ways of short DNA oligo to prevent further extension by polymerase during PCR?

I usually add 3' inverted dT modification on ssDNA probes to prevent further extension during the amplification process, but now I want to use some other cheaper modification to achieve this goal....

10 October 2022 2,339 0 View

Probe-based Melting Curve Analysis No Template Control has a peak? what reactions can happen between 3‘ modified fluorescent probe and polymerase?

I have a stubborn melting peak in my NTC line when I do probe-base one-step qpcr melting curve analysis for detection of RNA. After I did some control experiments, I found this issue is because...

10 May 2022 8,876 3 View