Zhou Wei

2 Questions 10 Answers 0 Followers

Questions related from Zhou Wei

One point mutant of gene, however the protein expression was enhanced about 80%. how to explain this phenomenon ?

The gene (1500 bp) was cloned into plasmid pET28a through NdeI/HindIII sites . a point mutant at 750 bp (G-A) was obained through error-prone PCR and the amino acid changed T-A. Then the...

02 March 2017 9,197 11 View

Are you familiar with a suitable method for E. coli acyl-CoA extraction and analysis?

I would like to extract and quantify acyl-CoA from E. coli. But it's difficult to extract because acyl-CoA are easily degraded by endogenous acyl-CoA hydrolase.

17 April 2015 2,410 2 View