Zhongtian Yu

4 Questions 3 Answers 0 Followers

Questions related from Zhongtian Yu

Purification of bacterial basic protein?

Hi researchgate, I am now attempting to purfiy some basic proteins from Streptomyces. I used E.coli as overexpression host. PI of these proteins are 7.14, 11.55 and 7.56. When I tried to...

26 May 2025 7,166 3 View

How should I change the resistance of a plasmid?

Hi everyone, I am now trying to delete some genes of the Agrobacterium Tumefaciens K84 strain. I decided to use the in-frame deletion method using the pk18mobsacB plasmid which harbours sac...

15 June 2023 8,990 1 View

Why can't my proteins be expressed?

Dear researchers, I am now trying to express several proteins from bacteria（Agrobacterium Tumefaceins) in one of its biosynthetic gene clusters. However, some of the proteins can be easily...

01 April 2023 622 4 View

Why can't I get the thermal shift result?

I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...

13 March 2023 1,281 1 View