Zhongtian Yu

3 Questions 3 Answers 0 Followers

Questions related from Zhongtian Yu

How should I change the resistance of a plasmid?

Hi everyone, I am now trying to delete some genes of the Agrobacterium Tumefaciens K84 strain. I decided to use the in-frame deletion method using the pk18mobsacB plasmid which harbours sac...

15 June 2023 8,943 1 View

Why can't my proteins be expressed?

Dear researchers, I am now trying to express several proteins from bacteria（Agrobacterium Tumefaceins) in one of its biosynthetic gene clusters. However, some of the proteins can be easily...

01 April 2023 569 4 View

Why can't I get the thermal shift result?

I am using Roche Lightcycler 96 machine to perform protein thermal shift assays. I added 15 uL of 50 uM protein and 5 uL of SYPRO ORANGE fluorescent dye in DMSO. The temperature ranged from 30 °C...

13 March 2023 1,182 1 View