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In mammalian cell fractionation experiments, it is often observed in protocols that after removing cytoplasm and nucleoplasm proteins, you digest your chromatin with either basemuncher or MNase...
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In Northern blot, we normally use either 2*SSC+0.1% SDS or 0.1*SSC+0.1% SDS in washing step. Does anyone know what's the function of the SDS in the washing buffer? Thank you in advance
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