Zhizhou Ye

3 Questions 5 Answers 0 Followers

Questions related from Zhizhou Ye

How to determine optimal antibody concentration for Sandwich ELISA using checkerboard titration method?

Hi All, I am optimizing concentration for detection 1' and 2' Antibody for a sandwich ELISA using the standard checkerboard titration method. (See attachment for experiment layout and specific Ab...

07 December 2018 6,776 2 View

Polysome profiling centrifugation parameters to separate polysomes better?

Hi, I have a question regarding the parameters (speed and length) during sucrose gradient centrifugation for polysome profiling. I'm attaching one typical chromatogram from my experiments. The...

19 October 2016 2,412 2 View

In a ribosome run-off assay with puromycin, is cycloheximide required (or shouldn't be added) during lysate preparation?

I'm doing a ribosome run-off assay in order to investigate the elongation status for one specific mRNA under two different conditions. In this assay I used puromycin to differentiate between...

03 October 2016 709 14 View