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Recently, I purify murine bone marrow neutrophil through 3-layer Percoll Gradients recently(72%-62%-56%), the purity is so low, between 40%-60%. I always follow the following steps:1. Grind the...
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I am doing the ROS production of BMDM from B6 mice, I usually plant the macrophages in 1*105 each well, wash with warm HBSS once and add 200ul HBSS with luminol (100uM) and 5U HRP each well,...
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