Zhenning Tan

4 Questions 9 Answers 0 Followers

Questions related from Zhenning Tan

My protein (PI 10) does not bind to cation exchange column efficiently?

I have been struggling with purification of a highly basic protein. This protein is His-tagged. We get pretty good purity from Ni column. The pooled Ni column fractions is in 500 mM NaCl, ~ 200 mM...

18 August 2016 2,860 8 View

How can I achieve high transfection efficiency for HepG2 cells?

I have used midi prep kit to purify plasmids and found that the transfection efficiency for HepG2 cells not very high, about 30%. I am using Turbofect from life technologies. My questions are, 1,...

18 May 2015 2,086 6 View

What is the recovery rate for preparing a plasmid vector?

I am cloning a vector from a 6 kb plasmid. I started with 10 ug plasmid. I did double digestion to remove 2kb fragment and then used phosphotase to treat the vector. After steps of digestion and...

16 March 2015 3,729 4 View

Can anyone suggest why I don't see a positive stain when immunostaining cultured cells?

I have been doing immunostaining of cultured cells. The current issue is that I don't see any positive stain. One thing that worries me is the fixation and permeabilization step. Is it possible...

22 November 2014 2,335 6 View