Thanks in advance! Molecular weight loss after HPLC purification and lyophilization, any experience or thoughts please?

I purified a small molecule with RP-HPLC using 0.01% TFA buffer. Before purification, LC-MS looks correct M+H around 726. However, after purification and lyophilization, M+H became 722. The...

19 February 2025 9,550 7 View