How to prevent aggregation during purifying DXS in protein?

I am purifying 1-deoxy-D-xylulose 5-phosphate synthase (DXS) enzyme in E. coli BL21 (DE3) cells. I used one-step nickel chromatography to purify it, but I analysed the sample in the SEC column,...

15 March 2024 1,925 5 View