Zhaowei Wu

3 Questions 10 Answers 0 Followers

Questions related from Zhaowei Wu

Is there anyone who knows where I can purchase anhydrotetracycline (aTc)?

I am currently working on a mutation generating in Staphylococcus aureus. However, This reagent, anhydrotetracycline (aTc), annoyed me. I can not found anywhere sell this reagent. Please help me!

13 August 2015 4,677 2 View

Is anyone interested in the methylation of E. coli?

I am reading the Molecular Biology of the Gene VI. I found that the initiation of genome DNA replication is triggered by the methylation of both double strands of oriC of E. coli. This mismatch...

09 May 2014 4,057 5 View

Pichia transformants lost target gene

Recently, I got problems about Pichia expression. My target gene was inserted into pPIC9K vector via EcoRI and NotI restriction sites. And then the SacI digested construct was transformed into...

01 March 2014 4,958 2 View