Zélie Dirand

2 Questions 3 Answers 0 Followers

Questions related from Zélie Dirand

How can I activate macrophages after differenciation, to induce their cytokine secretion ?

The aim of my study is to cultivate different types of macrophages, and to put them in co-culture with fibroblasts. I already arrived to differentiate monocytes in M1 and M2a macrophages. But my...

09 February 2022 1,689 2 View

Why do my differentiated THP-1 cells looks like macrophages but do not express their specific markers ?

I would like to be able to differentiate THP-1 into macrophages of several types, in particular M1 and M2a. To do this, I activated THP-1 cells with 200 ng/mL PMA for 72h, than I differentiated...

07 October 2021 132 4 View