Zeinab Aboezz

6 Questions 6 Answers 0 Followers

Questions related from Zeinab Aboezz

What could be problems contributed for the following indirect check board ELISA?

Hi, Could you help me for solving the problem for the following ELISA ( Check board ELISA) ? Although the serial double fold dilution of the Ag ( purified protein) and the serial double fold...

03 November 2022 8,687 6 View

Protein estimation for the trapped and non trapped protein in bilosome around 50ug/ml ?

Hello, I am trying to entrap some of viral protein inside bilosomal vesicle , I want to know the entrapment efficiency for my bilosome so I got them and separate the trapped one from non...

14 July 2022 9,360 0 View

What is the meaning of tripartite leader sequence in mRNA?

hello, every one any one know what is the meaning of Tripartite leader sequence for mRNA in adenovirus transcripts or generally for gene transcription . thanks zeinab

16 September 2016 5,200 3 View

What is the suitable program for cDNA sequence analysis ?

Hello everyone, I want know the program that I can use to analyze my Data sequence of part of cDNA inserted on plasmid to get information about the 5 untranslated region of the mRNA, to get more...

23 August 2016 3,630 3 View

How can i get rid off non specific bands in pcr reaction for CDNA?

Hello, every one if i have the following result how can get rid off nonspecific band if you know it is appear only with 2 tested sample as in lane 3,4 of my cDNA , but not appear with control...

05 July 2016 9,641 6 View

Can any one identify this lower band for my control -ve reverse transcription ?

Hello,every one I cleaned up my RNA samples with DNase1 , I made RT-pcr to make sure that sample become free from genomic DNA ,but i had in my gel result a smear in the distal part of 2 lanes as...

17 June 2016 4,715 3 View