Why don't I get any positive colonies in ligation of FatB into pCAMBIA1302?

Hi everybody, I am trying to ligate FatB to pCAMBIA1302 after digestion with Bgl II and BstEII. From pCAMBIA1302, mgfp5 fragment was removed after digestion. The concentrations of FatB and 1302...

22 October 2014 3,226 14 View