Zahran Somaya

8 Questions 10 Answers 0 Followers

Questions related from Zahran Somaya

Cardiac troponin I detection with sandwich ELISA

I'm working on detecting different fragments of cardiac Troponin I (Not using a kit) in the patients' sera of different cardiac diseases. I'm using the best antibodies recommended by Hytest (19c7...

03 March 2016 3,038 3 View

How can I use 2 capture antibodies to detect cardiac Troponi I, in sandwich ELISA?

Just wondering how I can stick the 2 antibodies to the wall of the plate? as I have them in separate aliquots. Should I incubate the first Ab O/N then next day incubate the other for another...

09 September 2015 7,133 3 View

Any insights with my sandwich ELISA development as it's not able to detect different concentrations of the protein in the sample?

I'm detecting Troponin I, using 19C7 for capturing, and M19 for detection. 1% BSA for blocking. I get signals with the troponin compared to the negative control, but it doesen't correlate with the...

04 April 2015 4,532 7 View

How can I identify the contaminating agent in ELISA?

I'm using 19C7 2ug/mL as capture, and M18 1 ug/mL as detection for Troponin I in ELISA, with SA-HRP. somedays, I get reliable results, and many others, it's all over the place :(( when...

04 April 2015 673 4 View

How can I improve the sensitivity of my ELISA?

I work on Troponin I, I use 19C7 as a capture, and M18 for detection both at 1ug/mL, I need the assay to detect down to 0.1 ng/mL, and I'm just getting signal at 50 ng/mL. I use 2% BSA for block....

04 April 2015 9,524 3 View

How do I prepare pre-coated Sandwich ELISA plates for later use?

I need help with making precoated ELISA plates, especially preserving. I never did this before, but it will save me a lot of time. What I found with google search is: incubate with capture...

04 April 2015 5,584 6 View

How can I detect or remove Nickel Hydroxide from my protein sample?

I cleave my protein from its vector using Ni sulfate under high temperature and PH, after lyophilizing. My protein came out yellowish, and it should be white, so I assumed that there are some...

12 December 2014 4,299 5 View

Why can't I get a pure protein with Ni column chromatography protein purification?

I have a problem with my protein purification, I'm using the Ni column chromatography, running my cTnI protein after cleavage from the His tag to get the protein without the His tag, but it's...

11 November 2014 8,336 7 View