Zahra Naseri

5 Questions 3 Answers 0 Followers

Questions related from Zahra Naseri

Can I visualize fluorescence exosomes in cryosection of tumor tissue by fluorescence microscope?

we have already encapsulated fluorescence siRNA into the exosomes and injected them in tumor bearing mice, after killing mice we prepared the cryosection from tumor tissue. Are these exosome be...

04 April 2016 8,974 4 View

Which freezing method is better for preparing cryosection so that the fluorescent effect be maintained?

I want to image nanoliposome conjugated-GFP in tumor tissue after intratumoral injection by fluorescent microscopy, which freezing method is better after removing of tissue for cryosectioning? Can...

02 February 2016 4,434 1 View

Do you know about the effects of exosomes isolation with PEG?

I isolated exosomes from supernatant of cell culture by PEG method, now i'm interesting about this issue that isolating exosomes with PEG may covering its targeting receptors? if this is true,...

01 January 2016 1,161 2 View

After hybrid formation between miRNA and inhibitor can I calculate target miRNA level?

I am working on miRNA inhibition with power inhibitors (exiqon) that not activate RNase H enzyme. after hybrid formation between miRNA and inhibitor can I calculate target miRNA level? because if...

11 November 2015 8,078 1 View

How can I measure efficiency siRNA loading into the exosomes?

I want to measure fluorescent labeled-siRNA loaded to exosomes with fluorimetry method? please describe me this method step by step? thanks

01 January 1970 5,540 2 View