Zahra Zangishei

6 Questions 8 Answers 0 Followers

Questions related from Zahra Zangishei

Does anyone know how to interpret the miRDeep2 output?

Hello I have used miRDeep2 to predict novel miRNAs in my small RNA datasets. The program generates a table with several columns like miRDeep2 score, estimated false positive, estimated true...

24 March 2020 829 2 View

how to best store collected tissues and maintain reproducibility between laboratories?

I am going to store my plant tissues for further experiments on (mRNA/miRNA-seq AND qPCR) later in another laboratory. Due to transportation process of my samples, so I am worry about maintaining...

01 June 2019 1,472 3 View

Any comment on my RNA non-denaturing gel?

I checked the quality of total RNA of tomato tissues following Trizol extraction on non-denaturing gel. I am planning to submit my samples for small RNA-Sequencing. What caught my attention is...

26 November 2017 520 2 View

In silico identification of miRNAs?

Hi everybody An easy issue that made me confused these days is why ESTs are used for in silico identification of miRNAs or why other types, for instance transcriptome seq data may not be suitable...

12 March 2017 3,670 3 View

How to get all plants miR/mir sequences from miRBase?

Hi every body I want to download all stem-loop and/or all mature miRNA sequences from all eudicotyledons and monocotyledons plants. How can I do that in miRBase 21?

26 January 2017 8,806 3 View

Is there any reliable computational miRNA binding site prediction programs?

I want to find probable miRNAs bind to and interact with my gene of interest. So, I think of predicting the putative binding sites of miRNAs on my target sequence. Is there any reliable...

14 January 2017 5,992 9 View