Zahra Sabouri

3 Questions 3 Answers 0 Followers

Questions related from Zahra Sabouri

What are the best protocols for making competent Bacillus subtilis cells and their transformation?

For my master thesis, I need to transfer Bacillus subtilis with a specific plasmid to edit its genome via CRISPR. I have some challenges with the existing protocols for making competent Bacillus...

01 February 2022 7,526 2 View

How to inactivated restriction enzyme if the enzyme can't be heated inactivated?

Dear all, Im new here and I need help from you all. How to inactivated restriction enzyme if the enzyme can't be heated inactivated? Such as sfiI from ThermoFisher. Please answer me. Thank you.

24 August 2021 901 5 View

How to design a primer for overlap extension pcr?

What is the length of the primer? What is the annealing temperature at each stage ?

29 May 2021 5,138 7 View