Zahra Mahmood

3 Questions 3 Answers 0 Followers

Questions related from Zahra Mahmood

Flow cytometric analysis to determine apoptosis in HT-22 Cells?

Hello, I am performing flow cytometry on knockdown HT-22 cells. I have attached a picture for reference as to what kind of results I am getting. I have used the "Annexin V-FITC Apoptosis...

26 July 2020 2,117 3 View

What to do if secondary structures found in transformed cellular sample?

Hello, I have inserted a 580 bp sequence in 3xFLAG plasmid. This sequence has a 66% GC content. PCR annealing temperature was high at 68C. Purification, digestion were fine. Transformation was...

27 March 2020 419 3 View

What Happens when Boiling Protein Samples Twice?

Hi. I prepared my protein samples. After boiling them I realized that i added less loading buffer to a couple of samples than i had calculated. But now its already boiled. Can i add more loading...

04 July 2019 4,278 4 View