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Questions related from Zahra Mahmood
Hello, I am performing flow cytometry on knockdown HT-22 cells. I have attached a picture for reference as to what kind of results I am getting. I have used the "Annexin V-FITC Apoptosis...
26 July 2020 2,148 3 View
Hello, I have inserted a 580 bp sequence in 3xFLAG plasmid. This sequence has a 66% GC content. PCR annealing temperature was high at 68C. Purification, digestion were fine. Transformation was...
27 March 2020 442 3 View
Hello. I performed PCR (used the Vazyme Phanta Max Super-Fidelity DNA Polymerase kit), ran the product (50 uL) on 1% agarose gel. Sometimes the band is of a normal size sometimes its thinner, (I...
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Hi. I prepared my protein samples. After boiling them I realized that i added less loading buffer to a couple of samples than i had calculated. But now its already boiled. Can i add more loading...
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Hi, I am working on the HPG axis. Is it possible to co-culture GnRH neurons with sperm cells? Or is there any other method that I can use to verify interactions between these two?
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