Zahra Hassani

5 Questions 7 Answers 0 Followers

Questions related from Zahra Hassani

Why can't I extract plasmids from bacteria DH5 alpha?

Hi I pTZ57R/T Fermentas vector to clone genes in the bacterium E. coli was used.Now I can not do of a single colony obtained plasmid extraction. I have 16 hours in a single colony was grown labial...

16 April 2015 1,182 6 View

Why can I not find any colony which contains the gene?

I have a 1300bp gene using vector psk my cologne. But after steps gene transfer colony can find her?After culturing the transfection bacteria in a medium containing IPTG and Xgal and...

13 February 2015 9,735 3 View

How to combat Beauveria bassiana fungus infection in my tobacco culture?

Hi All my tissue culture infected with the fungus.(Culture tobacco)I also used to combat fungal antibiotic Dodin but I had no effect

03 January 2015 1,756 10 View

Why can not ligation and transfer gene transfer in bacterial colonies can never take?

I do Cloning: in ligation: 15 µ DNA 5 µ Plasmid (pks), 2/5 µ H2O, 0/5 T4, Buffer 2µ Added and 90 Minute at a temperature of 16 ° C gave. The resulting product is then transferred into competent...

27 December 2014 1,134 5 View

What can I do about the concentration of DNA extraction bacteria without the use of NanoDrop and spectrophotometry determination?

How can I do agarose gel electrophoresis, and then compare the bands to the gene ladder and detect the concentration of the gene?

07 December 2014 9,898 9 View