Zahir Shah

7 Questions 31 Answers 0 Followers

Questions related from Zahir Shah

Functional assay for Dendritic cells In vitro?

I am looking for recommendations on the functional assays for Dendritic cells (DCs) in vitro. Could anyone suggest what viral plasmid is more potent to induce adaptive immune responses of T cell...

13 October 2020 3,453 1 View

How to distinguish the human primitive macrophages from definitive

I am wondering about the combination of cell surface markers e.e. Cd14, Cd11b, Cd33 and HLA-DR to use for the macrophages (yolk sac and definitive). Could anyone suggest the combination of these...

11 May 2017 5,379 1 View

To use P2A system for multiproteins production in hESCs, do I need to remove the stop codon of first protein and then add the P2A sequence?

I want to design a construct and to know the expression of resistive gene by fluprescence protein. So, I need to use the P2A system for this reason. The question is, whether I should remove the...

06 August 2016 518 2 View

How can I elute the His-tagged protein in non-reducing condition without using an imidazole buffer?

I am purifying my protein using imidazole, but imidazole interfere with my results and I want to use another solvent to elute protein. Can anybody suggest me another solvent with which I can get...

12 May 2014 5,260 48 View

How can I overcome the high uniform background in western blot?

I am getting a high background problem with western blot. I have given long time for blocking and washing with 1% TBST thrice for 10 minutes. But the result does not improve and the background is...

06 March 2014 4,531 6 View

Less Opa1 isoform bands after Western Blotting?

I have performed Western Blotting many times for getting the five bands for opa1 isoforms in HeLa and HeLa rho zero cells by running the samples on 12,15 and 18% gel for 8,10,12,14,16 and 18 hrs....

13 December 2013 4,701 2 View

Can anyone help with protein-protein interaction?

I am doing CoIP to determine the interaction of my protein of interest (Flag tagged) with another protein. I used the primary Ab against Flag and after WB, the result was okay but at the same time...

04 December 2013 3,932 3 View