Zahidah Zafhian

3 Questions 3 Answers 0 Followers

Questions related from Zahidah Zafhian

Can anyone help with my standard curve result on RT-PCR?

Hello all, I have questions about standard curve for absolute quantification..For template,I have done 1:10 dilution, (1 ul template and 19 ul sterile ddH2O) in 5 points which is template...

26 May 2015 6,738 12 View

Can anyone give me an idea on how I can isolate gDNA?

I have extracted the genomic DNA and purified it by using CTAB method. I would like to amplify my genomic DNA by using PCR method.Can anybody share which PCR method do I need to used since Ive...

09 April 2015 4,627 8 View

Is there any method to isolate genomic DNA of a gene?

I have isolated full length cDNa of squalene synthase gene from root of tongkat ali. however I would like to validate my result whether the gene have intron or not. so I would like to isolate the...

27 February 2015 7,733 5 View