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02 February 2018 5,267 1 View
I am adding an inhibitor to the enzyme. The Vmax decreased with increase of inhibitor concentration.The mechanism found out, showed a "mixed type of enzyme-inhibition". It was hypothesized that...
12 December 2015 1,612 3 View
In an enzyme assay to determine Vmax and Km, I varied the substrate concentration in uM. The extinction coefficient is used to convert the absorbance into the conc unit. Extinction coefficient has...
01 January 2014 3,346 13 View
I am studying the activities of Laccase (EC 1.10.3.2) enzyme in soil. Using ABTS (2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid). I cannot observe change in absorbance at 420nm or near...
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