Zack Saud

3 Questions 5 Answers 0 Followers

Questions related from Zack Saud

What may cause circles of cell death in centre of vero cell SARS-CoV-2 plaque assays?

Hi all, I have been doing plaque assays of SARS-CoV-2 using Vero E6 cells modified to express ACE2 and TMPRSS. Cells are plated at 10^5 in one mL 24 hours before in 12 well plates. Upon staining,...

01 December 2020 5,346 4 View

What is the optimum copy number of starting DNA template for PCR?

I understand the reasons why too much starting template can inhibit the formation of PCR amplicons, and I am aware of the guideline amounts of DNA in weight/volume measurements. But obviously the...

07 May 2017 5,795 5 View

Anyone know of a good online resource which allows automatic annotation of whole-exosome sequencing data (ie flags up unhealthy genes in data)?

Websites similar to www.promethese.com but for Illumina Hiseq NGS data.

18 February 2016 6,757 6 View