Bizzare qRT-PCR amplification curve - Why does signal drop to zero near end of run?

Sorry for the poor image quality, I am working with a fairly old real-time PCR system (Bio-Rad MyIQ on iQ 5 version 2.0 software). I am using primers, cDNA, and supermix (Bio-Rad iQ SYBR Green)...

08 August 2018 2,216 5 View