Zachary T Kelleher

2 Questions 6 Answers 0 Followers

Questions related from Zachary T Kelleher

Western blotting: got better bands after stripping blot; why?

overall:I ran a western on eluate from an IP. (3 gels). I ran eluates on Bio Rad TGX gel in 1X Tris glycine running buffer. Transfered in 1x Tris glycine Transfer buffer with 15% methanol, (using...

11 November 2019 7,902 2 View

Goal: run eluted protein that was reduced and denatured, and then frozen. How best to run?

I IP'd biotynylated protein with avidin beads. I washed the beads and eluted in Laemmli buffer +DTT. I heated to 95 C for 10 minutes. I then froze to samples at -80 to run a few days later. Is it...

10 October 2019 6,185 2 View