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Questions related from Zachary T Kelleher
overall:I ran a western on eluate from an IP. (3 gels). I ran eluates on Bio Rad TGX gel in 1X Tris glycine running buffer. Transfered in 1x Tris glycine Transfer buffer with 15% methanol, (using...
11 November 2019 7,909 2 View
I IP'd biotynylated protein with avidin beads. I washed the beads and eluted in Laemmli buffer +DTT. I heated to 95 C for 10 minutes. I then froze to samples at -80 to run a few days later. Is it...
10 October 2019 6,192 2 View