Zachary mullin-bernstein

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Questions related from Zachary mullin-bernstein

Adding a transcriptional repressor before a promoter sequence in my plasmid?

I am performing a KI experiment using CRIPR/Cas. Our 5' homology arm is the 5'UTR of the gene of interest and therefore contains the endogenous promoter. I am looking for a repressor sequence to...

20 May 2020 5,249 3 View

How to design a vector that uses loxP for removal of blasticidin resistance gene?

Using CRISPR/Cas9 and homoly directed repair I have knocked in a degron tag onto the start of a protein of interest in 293T cells. WB has confirmed that ~5% of protein alleles have recieved the...

18 March 2020 4,633 1 View