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Questions related from Zachary mullin-bernstein
I am performing a KI experiment using CRIPR/Cas. Our 5' homology arm is the 5'UTR of the gene of interest and therefore contains the endogenous promoter. I am looking for a repressor sequence to...
20 May 2020 5,226 3 View
Using CRISPR/Cas9 and homoly directed repair I have knocked in a degron tag onto the start of a protein of interest in 293T cells. WB has confirmed that ~5% of protein alleles have recieved the...
18 March 2020 4,608 1 View
