Zach Troyer

3 Questions 2 Answers 0 Followers

Questions related from Zach Troyer

Weird black spots on western blot... potential causes?

I ran BeWo lysate samples on a polyacrylamide gel in SDS under reducing conditions. Gel was transferred to nitrocellulose membrane and blocked with 5% nonfat milk/PBST. Primary and secondary...

01 January 2020 8,702 5 View

Can I use the immunodominant ovalbumin peptides in my splenocyte feeder cells when using ELISPOT to measure Ova-specific response?

Hello all, I'm using ELISPOT to measure the response of lymphocytes from mice immunized with full ovalbumin protein. Would I need to pulse my splenocyte feeder cells with full ova protein or can I...

07 July 2017 6,004 1 View

Is NEAA an essential component in the media for HEK-293s?

I've seen conflicting reports on whether or not MEM Non-essential Amino Acids is needed as part of the complete media for HEK293s. I know we grow HEK293Ts in the lab without it.

02 February 2016 396 2 View