Yuxian Lei

3 Questions 1 Answers 0 Followers

Questions related from Yuxian Lei

Why does empty plasmid after ligation showed different size band from positive control?

Hi all, I did in-fusion cloning trying to insert some fragments into a 7kb vector. After transformation and isolation of plasmid, I conducted test digestion using enzyme XbaI and ran positive...

17 April 2020 1,576 0 View

Why did test digestion by restriction enzymes result in larger than expected bands?

Hi all, I did ligation, transformation and minipreps, and then test digestion (EcoRI) to see if the inserts were successfully integrated in the plasmid. If the insertion were successful, I should...

16 April 2020 3,580 3 View

Why there's a higher than expected band when loading uncut plasmids?

Hi all, I was doing plasmid double digestion and got a weird result. The uncut plasmid should be 7kb. I expected to see 3 bands--nicked, linear, and supercoiled. However, I only got one band...

15 April 2020 4,916 3 View