Yuthika Dholey

2 Questions 2 Answers 0 Followers

Questions related from Yuthika Dholey

How to remove percoll for sample preparation after ultracentrifugation of the sample in percoll gradient?

I'm trying to isolate Lysosome using a percoll (sigma, cat# 4937) gradient of 57.6%, 43.2%, 28.8% and 20.2% (Ref: Lysosomal and non-lysosomal peptidyl hydrolases of the bloodstream forms of...

22 February 2024 1,012 1 View

Can anyone tell me what type of problem arose during the bacterial transformation after ligation on this kanamycin plate?

I am trying cloning of my gene of interest in pet28a vector and trying to transform it in DH5a. But after transformation I got this type of plate. Can anyone tell me what is the problem here?

13 January 2024 6,695 4 View