Yusuf Hussain

4 Questions 5 Answers 0 Followers

Questions related from Yusuf Hussain

How can we induce hepatocellular carcinoma in wild type animals (i.e C57BL/6 mice or Swiss albino mice or sprague dawley rats ) ?

Hello everyone, I am trying to develop animal model for Hepatocellular carcinoma (HCC) in wild type rodents such as C57BL/6 mice or Swiss albino mice or sprague dawley rats because of non...

06 June 2018 9,332 0 View

Can anybody suggest me how to design primers of a gene whose nucleotide sequence is not present on NCBI?

I am evaluating hepato-toxicity of drugs in mice (Mus Musculus). Considering cytochrome 450 gene subtypes CYP2C19, 2C9, 2D6, 2E1 1A2 and 3A4. I got nucleotide sequence of some genes from NCBI and...

03 March 2018 9,724 4 View

What protocol should be followed in Western Blotting for BDNF like small proteins in in-vitro glial treatment?

I am doing drug treatment in primary culture of astrocytes, in western blotting big proteins like GAPDH and Actin are prominently detected but smaller proteins like BDNF(17kda), GDNF(17kda) and...

07 July 2016 1,894 3 View

Why are female rats are more prone to addiction?

I am looking for reasons why female rats are only used for addiction models and which neurotransmitters and neuropeptides are responsible for it and brain region where it works.

07 July 2014 519 2 View