Why my target protein can be induced when BL21 grow slowly and not be induced when grow slowly?

I insert about 600bp cDNA of a gene in pET28a-His-SUMO plasmid, and trying to express this fused protein in BL21 STAR. But I can not induce this protein to express everytime. And I found a regulor...

28 August 2020 4,153 3 View