Yunlong Jia

4 Questions 7 Answers 0 Followers

Questions related from Yunlong Jia

It's possible for Lenti-virus infection in T75 falsk ?

Recently , I am doing lentivirus-infection in HEK cells. According to the protocol of BROAD Institute: "https://portals.broadinstitute.org/gpp/public/resources/protocols", the infection always did...

03 March 2019 1,581 3 View

Gateway efficiency about LR reaction ?

Does someone know about Gateway efficiency? I mean, if I use 150ug DONR plasmid and 150ug Destination plasmid, finally, how many plasmid really take place the LR reaction ? or percentage? I've...

10 October 2018 9,510 2 View

Which ORF is real ORF in the plasmid, how to choose the right one ?

Hi, dear colleagues, Recently, I really confused about ORF in plasmid. I checked my target sequence in my plasmid, in fact, after the promoter there are two possible ORFs, 1st ORF is close to my...

07 July 2018 6,148 3 View

How could rop gene be expressed in plasmid?

Now, I am working on pDest-565, here is its feature map in addgene: https://www.addgene.org/11520/ I want to know how rop gene [6698-6889bp] could be expressed. In fact, on the upstream of rop,...

02 February 2018 279 5 View