Yumin Huang

2 Questions 1 Answers 0 Followers

Questions related from Yumin Huang

How to explain that the length of transfected gene become larger in the cells?

Based on my previous question on ReseachGate, I transfected the gene of Norepinephrine Transporter into human colon cells. The sequence of the vector has been confirmed correct. Usually, after...

20 June 2024 1,027 3 View

Why the PCR product turns bigger after transfection into cells while using the same primer?

I wanted to check the expression of a transfected membrane protein called NET, so I decided to check the mRNA by RT-PCR. After three months of culture selection (the cells were still not...

19 June 2024 5,764 3 View