Yumi Nakayama

4 Questions 9 Answers 0 Followers

Questions related from Yumi Nakayama

APC channel positive with unstained cells?

One of my cell lines shows up as positive in APC channel without any APC conjugated antibody by flow. I only have two colors which was stained separately so none of the samples have both APC and...

20 November 2017 5,203 3 View

Hello, does anyone have an experience with two peaks of Aiolos in B cells by flow? I get one or two peaks sometimes but it should be one peak right?

I use transcription factor buffer set from BD. I have not changed anything but I started to get two peaks in my Aiolos staining in B cells. I got two peaks for another transcription factor which...

29 August 2017 6,286 3 View

Any tip to improve BC in my human PBMC culture? It always dominates with T cells, very low percent of B cells and almost no monocytes?

I use 10% human serum, 1% penicillin streptomycin RPMI 1640. Isolate PBMC from buffy coat and culture with or without stimuli. T cells are 60-70% after two days and 1-7% B cells, no monocytes by...

20 December 2013 6,635 4 View

SEB stimulation on purified CD4 or CD8 T cells.

Hello, I have used SEB for T cell stimulation but SEB binds to MHC II and TCR, why is it so potent to simulate purified T cells not PBMC without any APC in it? T cells do not express MHC II right?...

01 January 1970 1,686 9 View