Yulia Steblyanko

3 Questions 12 Answers 0 Followers

Questions related from Yulia Steblyanko

Can I use nucleofection in hESCs during differentiation protocol?

Hi guys, I'd like to do siRNA transfection/nucleofection during different stages of hESC differentiation. Nucleofection should provide the better efficiency of siRNA expression. However, the...

25 May 2022 2,163 2 View

In what color Toluidine Blue O (TBO) stains xylem and phloem plant tissues?

Dear Community, Can you please help me by answering this basic question? I was confused with the answers I found on the internet. Some sources say that xylem stains red and phloem stains blue by...

04 December 2020 3,714 8 View

Wrong or no fragment insert in directional TOPO cloning?

Dear experts in molecular biology, I am having trouble cloning my PCR-amplified ~4.4kb fragment into the pENTR/D-TOPO vector (2.58kb) directional cloning vector from Invitrogen. I am using a...

18 July 2016 10,278 10 View