Yulia Ermakova

3 Questions 7 Answers 0 Followers

Questions related from Yulia Ermakova

Native protein complex IP: should I use frozen or intact cells?

Dear all, I need to make a protein immunoprecipitation (IP) to find partners of the tagged protein in mammalian cell culture. I see in >90% of protocols people use frozen cell pellets. The...

21 May 2022 8,789 0 View

What is the effiency of co-transformation of 2 plasmids in bacteria?

I have to make a screen, based on 1 hybrid assay in bacterial cells. Technically, I just need to co-transform 2 plasmids, the substrate, and the binding protein plasmid and select for the...

15 March 2021 7,853 10 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

07 January 2021 7,347 2 View