Yuka Takemon

3 Questions 8 Answers 0 Followers

Questions related from Yuka Takemon

Is my RNA degreding during the Northern blot transfer step?

Hi, I am currently trying to run a Nothern blot to confirm a possible mutant that we have, and along with this a wild-type sample as the positive control sample. I am using the Ambion...

05 May 2015 3,622 11 View

Leaving PBS during cell disassociation. Is this a problem?

I have been using Accutase as a trypsin like enzyme to dissociate adherent cells in a flask. To split the cells I first decant the media and rinse them with PBS, but after the rinse I added fresh...

08 August 2013 4,707 3 View

How to use ENCODE predicted lncRNA to find exact edges of the transcript?

I have single/multiple long ncRNAs that were predicted by ENCODE. As you know ENCODE layers transcripts to build a peak (bell-shaped curve) indicating its level of expression. How do you go about...

05 May 2013 9,238 6 View