Yuhang Shi

2 Questions 4 Answers 0 Followers

Questions related from Yuhang Shi

Does anyone have experience on using L-mimosine and Thymidine in 293T cells?

I am working on 293T cells to check effects of some treatments on the cell cycle distribution by flow. Thymidine (Sigma) is synchronizer I used to block cell cycle in G1 phase. L-mimosine (Sigma)...

29 January 2020 1,787 4 View

How to make Thymidine solution and How to perform thymidine double block in 293T cells?

I am doing thymidine double block in 293T cells to arrest cell cycle in G1/S phase. But the results in FACS were not expected and only around 20% of cells stay in G1 phase. The protocol I followed...

07 November 2019 9,360 3 View