Yuhan Lyu

4 Questions 4 Answers 0 Followers

Questions related from Yuhan Lyu

There is stop codon of the sequencing of my phage library construction with hyper-phage?

I used pHEN2 phagemid and CM13d3 hyper phage to construct a phage library with 5 randomized amino acids. When I sequence the library, there is stop codon showed up within the 5 randomized aa area....

14 July 2022 3,739 0 View

Phage library construction (phage vector vs phagemid vs helperphage)?

This is a fundamental question about basic phage library construction. I know that people can construct library by either phage vector (eg. M13KE) or phagemid+helperphage. Based on my...

04 December 2021 8,648 4 View

Can I construct a phage library to display disulfide bond constrained mini protein?

I am working with phage displayed library construction. Our lab constructed pIII-displayed peptide (CX6C, CX9C, CX12C) library using M13KE (Ph. D. Cloning system NEB #E8101). Instead of randomized...

13 October 2021 798 2 View

Facilitate disulfide bond formation after expression?

Hi, I am expressing a MBP-fusion protein with 4 cysteine and I see free cysteine on LCMS indicating the disulfide bond formation is not complete while expression. Is there any way to facilitate...

18 September 2021 449 5 View