Yue Li

5 Questions 9 Answers 0 Followers

Questions related from Yue Li

Should I change parameters for cycle sequencing, when the primers' annealing temperature is lower than 50 °C?

Hi colleagues, The cycle parametre for cycle sequencing of Bigdye Terminator V 3.1 cycle sequencing Kit is "96°C 1min; 96°C 10s, 50°C 5s,60°C 4min, repeat 25cycles; 60°C 4min". Some of my primers...

13 September 2015 7,590 1 View

How many variations (nucleotide mutations) are necessary to construct TCS network using chloroplast regions from plant species?

Hi colleagues, I try to screen some chloroplast universal primers to construct TCS network (and maybe cp haplotype phylogenetic tree and divergent time for each code) for one species. I read some...

14 July 2015 4,435 3 View

When perform multivariate analyse, can I use negative correlated variables?

I understand positive correlated variables cannot be used in multivariate analyse (such as, PCA). How about negative correlated variables? Can they be used in multivariate analyse? Thanks.

02 March 2015 5,752 4 View

When using MaxEnt, how can I test multicollinearity of environmental variable?

Hi colleagues, I have a set of occurrence data of a species (about 50 localities). And, I want to use these data to estimate the the potential distribution of the species by MaxEnt. I downloaded...

28 February 2015 2,701 15 View

How can I extract environment data from GIS layer for locality point?

I downloaded bioclimatic variables from WorldClim. I want to extract environment data for random or specific locality point and convert the data to ascii format? Can anyone guide me? Thanks in...

28 February 2015 9,960 7 View