Yuanjian Huang

3 Questions 4 Answers 0 Followers

Questions related from Yuanjian Huang

Is it possilbe to knock out only one allele in zygote?

My way is to knock out one gene in sperm and inject this sperm into a normal wild-type ovum. So, theoretically, it will produce the genotype and phenotype with only one allele knocked out. Is it...

25 February 2020 6,887 0 View

How could the gene on plasmid express the right protein in cells?

Hi guys, A gene will be translated into a hnRNA fisrt. After processing like cutting the introns, hnRNA will become mRNA in eukaryocyte cell. When we want to establish a plasmid carrying this...

01 January 1970 4,813 3 View

Transient transfection of gRNA into stable Cas9 cell, will the knock-out be permanent？

Is the knock-out permanent if I transient transfect gRNA into a stable Cas9-expressed cell line? I think it will be permanent knock-out because once the indel is created, it is irreversible...

01 January 1970 5,058 2 View