Yuan Zhang

4 Questions 8 Answers 0 Followers

Questions related from Yuan Zhang

What is the difference between series dialysis of 8M urea and one-time dialysis?

I tried 8M Urea buffer for plant protein extraction and nickel purification. I equilibrated the nickel beads with TEV cleavage buffer which contains 50mM tris, phosphate, 300mM NaCl. Then I added...

03 March 2017 5,792 7 View

Once proteins purified, should I worry about degradation?

I purified my proteins from plants in 7M urea buffer through nickel purification. Then I changed purified proteins into a native condition buffer for overnight (Tris and phosphate) for other...

03 March 2017 6,289 6 View

How many proteins in a rice cell?

I noticed a drastic expression difference for the same protein between Arabidopsis and rice. I am wondering why. Though I used equal amount plant material for immunoblotting, my protein abundance...

01 January 2017 3,492 3 View

Why my transgene does not express as high in T4 generation as early generation in Arabidopsis??

I just obtained homozygous line of one of my overexpression lines. I chose this line for propagation because its expression was highest among other transgenic plants when I use western blotting to...

11 November 2016 3,621 5 View