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Questions related from Yuan-Yuan Cheng
I aim to compare the transcription level of two genes that encode two enzymes with the same function. I extracted mRNA and compared their transcription using RT-PCR. The Ct (also called Cq) values...
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Does anyone use TetR-based Tet-on expression system in bacteria, such as E.coli ? I used 100 ng/ml doxycycline to induce expression for one hour. However, I haven't observed difference between...
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I found a bacterial transcription factor X proved by EMSA. Transcriptome showed that some genes' expression are sigenificantly altered by X. And I wonder if it is possible to predict the conserved...
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An operon in a bacterium contains several genes. The transcriptional level of first gene is ten-folds higher than following genes. Possible reaons for this?
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